The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’ genes :

1. A. The gene ‘X’ is responsible for resistance to antibiotics and ‘Y’ for protein involved in the replication of Plasmid.
2. B. The gene ‘X’ is responsible for controlling the copy number of the linked DNA and ‘Y’ for protein involved in the replication of Plasmid.
3. C. The gene ‘X’ is for protein involved in replication of Plasmid and ‘Y’ for resistance to antibiotics.
4. D. Gene ‘X’ is responsible for recognitions sites and ‘Y’ is responsible for antibiotic resistance.

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